ABSTRACT
Objective To investigate the value of F-actin autoantibodies in the serum of patients with systemic lupus erythematosus (SLE),and to explore the relationships between F-actin autoantibodies and other clinical indicators.Methods ELISA was established to detect serum levels of F-actin autoantibodies in 93 inpatients with SLE from March 2017 to January 2018 (case group,n=93),72 patients with rheumatoid arthritis (RA) (disease control group) and 83 healthy subjects (healthy control group) were included during the same period.The positive rates of F-actin autoantibodies between the case group and the two control group were compared.Clinical data including SLE disease activity index (SLEDAI),immuno-globulin (lg)G,erythrocyte sedimentation rate (ESR),anti-dsDNA,and antinuclear antibody (ANA) of 93 patients with SLE were collected and the correlation analysis between F-actin autoantibodies units was applied respectively.The diagnostic performance of F-actin autoantibodies in SLE was analyzed by using the receiver operating characteristic curve (ROC).T test,Chi-square test and Spearman/Pearson correlation analysis were applied for statistical analysis.Results The serum levels of F-actin autoantibodies in the SLE case group,disease control group,and healthy control group were (18±13),(12±6),and (11±5) U,respectively,the differences between SLE case group and disease control group,and healthy control group were significant (t=3.163,P=0.001 9;t=4.436,P＜0.01).The positive rates of F-actin autoantibodies were 33％(31/93) in patients with SLE,10％(7/72) in disease control group,and 4％(3/83) in healthy control group.The F-actin autoanti-bodies units in SLE were correlated with SLEDAI,IgG,ESR,anti-dsDNA,and ANA (r=0.273 7,P=0.008 3;r=0.558 7,P＜0.01;r=0.419 9,P=0.000 1,r=0.351 4,P=0.001 1,r=0.460 9,P＜0.01),in which F-actin autoantibodies units showed significant correlation with IgG and ANA.In the ROC curve,the area under the curve(AUC) was 0.62 [95％CI(0.54,0.70)],P=0.001 3.which was statistically significant.When the cut-off value of the F-actin autoantibodies was 14.04 U,the Youden's index (YI) was the largest (YI=0.30),and the sen-sitivity for the diagnosis of SLE was 0.77,the specificity was 0.53.Conclusion The positive rate of F-actin autoantibodies in the serum of patients with SLE is higher than that of RA and healthy controls,so it has certain diagnostic value for SLE.The F-actin autoantibodies units is correlated with both SLEDAI,ESR,and anti-dsDNA,suggesting that F-actin autoantibodies units may be a new biomarker for disease activity assessment of SLE patients.
ABSTRACT
Objective To investigate the effect and significance of Jiangu granule on the expression of aquaporin 3 (AQP3) in articular cartilage of knee osteoarthritis (OA) rats.Methods Sprague-Dawley (SD) rats were randomly divided into sham operation group, model group, and Jiangu granule treatment group.The treatment group was divided into Jiangu granule high dose, medium dose, and low dose groups, with 12 rats in each group.The osteoarthritis model was made by injecting 4％ papain and 0.03 mol/L Lcysteine mixture into the knee joint.Sham-operated group and model group received normal feeding every day.Treatment group (high, medium, and low dose groups), the next day after the completion of the mod el, were given different dose Jiangu granule by gavage twice a day for 4 weeks.After treatment, the ratswere executed uniformly, using general optical microscope and the pathological section of histology to observe the articular cartilage damage.The expression of AQP3 in articular cartilage of rats was detected by real time polymerase chain reaction (RT-PCR) and Western blot.Results The articular cartilage destruction of Jiangu granule treatment group was significantly less than that of the model group (P ＜ 0.05).The mRNA and protein expression levels of AQP3 in model group was significantly higher than that in the sham operation group (P ＜ 0.05).OA rats with Jiangu granule after 4 weeks treatment, the mRNA and protein expression levels of AQP3 in articular cartilage were decreased significantly.Jiangu granule in each sub group relative to the model group had statistical significance (P ＜ 0.05), and the high dose group was decreased significantly.Conclusions Jiangu granule can reduce the expressions of AQP3 in OA articular cartilage of rats and alleviate the swelling of joints, which may have protective effects on articular cartilage.
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Objective To explore the changes of TGF-? and GM-CSF in rats with experimental bronchial asthma of spleen-deficency and the regulatory effect of acupuncture at Zusanli. Methods The rat models with spleen-deficiency in TCM and bronchial asthma in West Medicine were set up. They were given acupuncture at Zusanli. The concentration of TGF-? and GM-CSF in BALF were detected by enzyme linked immuoabsorbent assay (ELISA) and apoptosis was detected by TUNEL. Results Compared with the asthma group, the count of EOS and the levels of GM-CSF increased (P